South Dakota has been invited to continue participating in a national soybean research effort initiated in 2021, examining biological N fixation with thirteen participating universities (Ignacio Ciampitti, Kansas State University; and Seth Naeve, University of Minnesota; Lead PIs). Biological Nitrogen Fixation may be the most important economic and environmental process affecting soybean production in the United States, yet it is only partially characterized. We know surprisingly little about the quantities of N fixed by US soybean crops or the fate of that N in the environment. A better understanding of the soil, soybean management, and environmental factors affecting BNF along with the quantities of N fixed will contribute to scientific knowledge leading to greater soybean yields, higher soybean quality, lower environmental impacts, and greater returns to US farmers.