Frogeye leaf spot has now become a serious threat to soybean production in Indiana and the North Central Region. It caused record yield loss in 2018. Resistant cultivars and other tools are needed to control this disease. Here we propose to investigate pathogen biology, especially the aspects closely related to disease control, and to develop breeding resources for this disease. Specifically, we have three objectives: 1) identify new sources of resistance to frogeye leaf spot; 2) characterize previously identified sources of resistance and identify associated markers to facilitate breeding; and 3) evaluate virulence spectrum, population structure, sexual recombination, and QoI-resistance in the pathogen, C. sojina.