2021
Visual rating for iron-deficiency chlorosis (2020)
Contributor/Checkoff:
Category:
Sustainable Production
Keywords:
GeneticsGenomicsSeed quality
Lead Principal Investigator:
Carrie Miranda, North Dakota State University
Co-Principal Investigators:
Project Code:
QSSB
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:
Iron Deficiency Chlorosis is a major yield-reducing soybean disease. This project compares IDC resistance of approximately 200 soybean varieties from commercial lines and the North Dakota soybean breeding program. The researchers will also assess farmers’ fields with known IDC issues over six weeks, where they rank the soybean plants based on visual symptoms. The ranks are averaged for an IDC-resistance number. They also take into account environmental factors such as drought or excessive rainfall. A soybean plant can recover from IDC, but yields will be reduced. These scores can help guide farmers when planting.
Key Beneficiaries:
#agronomists, #Extension crop specialists, #farmers, #soybean breeders
Unique Keywords:
#idc, #iron deficiency chlorosis, #soil fertility, #soybean diseases, #soybean varieties
Information And Results
Project Summary

Iron-deficiency chlorosis (IDC) is common in North Dakota and reduces yield. The best way to reduce the yield losses associated with IDC is to select a tolerant cultivar. Growers need data that compares varieties from all the different companies in the same locations. This proposal would permit approximately 350 different GMO company varieties and non-GMO varieties to be evaluated for IDC tolerance. An additional 100 NDSU breeding lines would be evaluated. All private company varieties that have been entered into the Langdon Research and Extension Center (REC), Carrington REC, Minot REC, and Fargo Main Station yield trials will be evaluated for visual ratings of IDC at multiple field locations. Visual yellowing that is caused by IDC has been shown to be closely correlated to yield for fields that have IDC. This data will provide an independent confirmation of the IDC tolerance of company products and enable growers to compare varieties from many different companies. This proposal will aid growers in selection of IDC tolerant cultivars to increase yield on fields that have a past history of IDC.

Project Objectives

The objective is to screen all private company varieties that have been entered into the Langdon
Research and Extension Center (REC), Carrington REC, Minot REC, Williston REC and Fargo
Main Station yield trials for visual ratings of IDC at multiple field locations with a past history of IDC symptoms. A second objective is to provide visual IDC screening of approximately 100 advanced NDSU breeding lines. Soybean growers need information to aid them in comparing varieties from many different companies for iron-deficiency chlorosis (IDC) in side-by-side comparisons. This data provides unbiased information that enables growers to choose the best variety for their IDC prone fields. Visual yellowing that is caused by IDC has been shown to be closely correlated to yield for fields that have IDC.

Project Deliverables

Growers will be provided with data that enables them to identify the best varieties for those fields that are susceptible to IDC. Also, data will be provided to aid the soybean breeder in the development of NDSU cultivars that are tolerant to IDC. Data will be analyzed and reported in the NDSU bulletin entitled ‘North Dakota Soybean Performance (A -843)’ and posted online at https://www.ag.ndsu.edu/varietytrials/soybean.

Progress Of Work

Update:
Visual rating for iron-deficiency chlorosis (2020) - Helms/Miranda

Objectives of the research: The objective is to screen all private company varieties that have been entered into the Langdon Research and Extension Center (REC), Carrington REC, Minot REC, Williston REC and Fargo Main Station yield trials for visual ratings of IDC at multiple field locations with a past history of IDC symptoms. A second objective is to provide visual IDC screening of approximately 100 advanced NDSU breeding lines. Soybean growers need information to aid them in comparing varieties from many different companies for iron-deficiency chlorosis (IDC) in side-by-side comparisons. This data provides unbiased information that enables growers to choose the best variety for their IDC prone fields.

Completed work: Hill plots were planted at three locations with a past history of IDC. Iron-deficiency chlorosis was observed at two of the three sites. Two-hundred and seven Roundup Ready® and Xtend soybean varieties were evaluated for visual symptoms of IDC at two sites where IDC was present. Thirty-three Liberty Link or non-GMO cultivars were evaluated for IDC at two sites where IDC was present. Ninety experimental lines or released cultivars that had been developed at NDSU were evaluated for visual IDC score. Each genotype was evaluated at two different growth stages on a 1-5 scale. At each site there were four replicates. Iron-deficiency chlorosis was evaluated at about the V3-V4 stage and again at the R1-R2 stage. The data was averaged across both of these rating growth stages. The data was of very good quality. The results were published online at https://www.ag.ndsu.edu/varietytrials/fargo-main-station/2020-trial-results/2020-ndsu-enlist-llgt27-roundup-ready-and-xtend-soybean-iron-deficiency-chlorosis-trial/view and will be printed in the Soybean Variety Trials bulletin A-843.

Preliminary results: The data was of good quality and the known check varieties performed as expected.

Work to be completed: This project is completed for the 2020 growing season. We are moving the application submission online to prepare for the 2021 season. Private companies will submit varieties into the state yield trials at any location in the state, seed of these will be packaged in preparation for planting in May or June of 2020. These entries will then be scored for visual IDC symptoms in June and July of 2020.

Final Project Results

Update:
July 1, 2020 – June 30, 2021 Annual Report: Visual ratings for iron-deficiency chlorosis

Principal Investigator: Dr. Carrie Miranda, Department of Plant Sciences, North Dakota State University

Soybean growers with fields that have a past history of IDC, need information to aid them in comparing varieties from many different companies for iron-deficiency chlorosis (IDC) in side-by-side comparisons. There are genetic differences among cultivars for tolerance to IDC. We measure the tolerance to IDC by the amount of yellowing in the leaves at sites with a past history of IDC. Visual yellowing that is caused by IDC has been shown to be closely correlated to yield for fields that have IDC. This data provides unbiased information that enables growers to choose the best variety for their IDC prone fields. This data enabled growers to compare varieties from many different companies. Comparing soybean varieties from different companies requires that the varieties of both companies are evaluated, averaged across the same fields. Otherwise, a fair comparison is not possible.

The objective was to screen all private company varieties that have been entered into the Langdon Research and Extension Center (REC), Carrington REC, Minot REC, and Fargo Main Station yield trials for visual ratings of IDC at multiple field locations with a past history of IDC symptoms. A second objective is to provide visual IDC screening of 104 advanced NDSU breeding lines.

In 2020, three locations on farmer-cooperator fields with a past history of IDC symptoms had been identified and were later planted with hill-plots. There were 206 GMO company varieties tested and 33 Liberty Link and non-GMO company varieties tested. Those locations included Leonard, Colfax, Erie, ND. A total of 2,868 hill-plots were rated for IDC. Each hill was hand planted with eight seeds and later thinned to three seeds per hill to provide a uniform plant density. The sites were either sprayed with glyphosate or hoed to eliminate weeds.

Each hill was rated two or three different times at different soybean growth stages to record the visual IDC score at the three-leaf stage and later as the plants recover from the IDC symptoms. The rating scale is 1- green, 2- slight yellowing between leaf veins, 3- extensive yellowing, 4- growing point is dead, 5- plants are dead. Each hill is rated to the nearest one-half rating value. Also, the NDSU soybean breeder evaluated 100 advanced NDSU breeding lines for visual IDC symptoms.

The 2020 data was very good and the check varieties performed as expected. The data was very good at Leonard and Erie sites and unusable at the Colfax site. The extent of IDC symptoms at a specific site is dependent on having sufficient rainfall at early vegetative growth stages. Each variety was averaged across four replicates within each of the four sites. Data was analyzed and reported in the NDSU bulletin entitled ‘North Dakota Soybean Performance’ and was posted online.

This is the largest data set with the most comparisons of many different company varieties, both Roundup Ready, Liberty Link and non-GMO for North Dakota and western Minnesota. Because the data is averaged across two-three locations with four replications per location, the data is quite reliable in aiding growers to select the best varieties for their IDC prone fields. This data enables growers to increase their yield on their IDC prone fields because those varieties with the least amount of yellow IDC symptoms will yield the best on those fields that have that problem.

July 1, 2020 – June 30, 2021
Final Project Results: Visual ratings for iron-deficiency chlorosis

Principal Investigator: Dr. Carrie Miranda, Department of Plant Sciences, North Dakota State University

Iron-deficiency chlorosis (IDC) is common in North Dakota and reduces yield. The best way to reduce the yield losses associated with IDC is to select a tolerant cultivar. Growers need data that compares varieties from all the different companies in the same locations. This proposal permitted more than 250 different GMO company varieties and non-GMO varieties to be evaluated for IDC tolerance. An additional 104 NDSU breeding lines were evaluated. All private company varieties that were entered into the Langdon Research and Extension Center (REC), Carrington REC, Minot REC, and Fargo Main Station yield trials were evaluated for visual ratings of IDC at multiple field locations. These data provided independent confirmation of the IDC tolerance of company products and enable growers to compare varieties from different companies. This project provides an unbiased comparison of yield data in the same environment for growers to select tolerant cultivars to increase yield on fields that have a past history of IDC.

In 2020, 206 GMO company varieties were tested and 33 Liberty Link and non-GMO company varieties were planted in three locations. Those locations included Leonard, Colfax, Erie, ND. Two locations successfully showed IDC symptoms and scores were recorded. Data was analyzed and reported in the NDSU bulletin entitled ‘North Dakota Soybean Performance’ and was posted online.

The goal of this work is to give growers additional data so they can make the best decisions when choosing varieties, especially on IDC affected soils. Support for this project is high among private companies as well, as nearly 250 varieties were entered in 2020. Fields infected with IDC were selected for the 2021 season during 2020. Due to the usefulness of the data collected, this project will continue into 2021.

Benefit To Soybean Farmers

This data will provide an independent confirmation of the IDC tolerance of company products and enable growers to compare varieties from many different companies with all of those varieties evaluated at the same locations. Comparing soybean varieties from different companies requires that the varieties of all companies are evaluated, averaged across the same fields, otherwise a fair comparison is not possible. This data enables growers to increase their yield on their IDC prone fields because those varieties with the least amount of yellow IDC symptoms will yield the best on those fields that have that problem.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.