Continuous dietary intake of foods with anti-inflammatory bio-activities is a promising approach to prevent chronic inflammation, one of the significant threats for health in the US citizen. Potential anti-inflammatory bioactivity in soybean sprouts has been revealed by the PIs in the Nebraska Soybean Board funded FY19 and FY20 research projects. The bioactivity should be largely depending on the availabilities of compounds, including phytochemicals and bioactive peptides in the gastrointestinal digests of the sprout, which are affected by the growth conditions of parental plants. In this FY21 project, the effects of heat and drought on the chemical composition of the soybean and further the anti-inflammatory activity of soybean sprout will be analyzed to assess the stability of bioactivity in the soybean derived diets.