Updated January 27, 2021:
Final Report
Chen/Mian/McHale/Mozzoni: Through this project, we were able to develop breeding lines with a “balanced” composition of protein and oil; one such line is in the last stage of testing and being ramped up for release as germplasm for the greater breeding community. In addition, the support from USB allowed the identification of new genetic sources that have been used in crosses to develop future germplasm.
McHale: A tool for estimated processing value of soybeans based on yield, protein and oil concentration was developed for soybean breeders to evaluate breeding lines for estimated economic value on a per acre basis. Continued work is being done to integrate the estimated processing value calculator (including automated sourcing of multi-year market prices from the Agriculture Marketing Service database) and selection index into an “R” based module for dissemination.
Clemente: This program is reducing to practice the synthesis of novel co-products in soybean seed, namely the production of very long chain omega-3 fatty acids EPA (DHA in progress), high value carotenoid astaxanthin, a form of Vit E (tocotrienol) along with oil elevated in stearic and oleic acids. These novel output traits hold great potential for applications in feed, food, industrial and cosmetic industries.
Alonso: Mature seeds, harvested in Fall 2019 from four different states were analyzed (total 244 samples) for their carbohydrate content to guide the breeders in our team. The workflow developed for carbohydrate quantification was published in the journal Molecules, and publically available (https://www.mdpi.com/1420-3049/25/17/3806). On the other hand, a new manuscript that highlights the differences between transgenic lines and the wild type is under preparation. Besides, new embryo culture conditions were developed; those not only meet the criteria of matching the growth in the plant, but also of uptaking substrates at a constant rate. Both requirements are mandatory for the application of the mathematical model necessary for understanding the carbon distribution in the embryo.