Identification of cultural management practices that can impact seed protein and essential amino acid concentrations is critical to increasing soybean nutritional value. Greater nitrogen (N) capture is required to increase yield and protein concentration concurrently. Thus, characterization of limitations to N uptake (both fixed-N and soil-N) will assist in establishing best management practices to improve soybean seed protein, its quality, and its economic value. The overall objective of this project is to identify factors affecting soybean seed protein accumulation and composition and to identify the best management practices for increasing soybean seed quality. This characterization will serve as a baseline by farmers and scientists upon which future progress on seed quality can be measured. The contribution of different cultural practices on soybean protein will be evaluated via 1) meta-analysis of current and past scientific data investigating changes in crude protein due to cultural practices; 2) multi-state research studies focused on exploring best candidate practices identified by the Phase 1 meta-analysis to characterize treatment impacts on seed protein and essential amino acids; and 3) surveys will be conducted in FY20 and FY21 to gain a better understanding of the initial and final status of soybean seed quality knowledge from the farmer, agronomist, stakeholder, and industry perspectives. This project will provide new information on how to increase seed protein and essential amino acid levels by implementation of specific cultural practices, improving knowledge and transferring this information to farmers in order to increase U.S. soybean value.