Update:
Soybean is affected by a wide range of diseases caused by fungal, bacterial, and viral diseases as well as plant-parasitic nematodes, resulting in reduced quality, yield, and profitability in recent years. This project targets soybean virus diseases as a problem faced by soybean production in the Delmarva region. In 2019-2022, a total of 184 soybean samples displaying viral disease symptoms, including mosaic, mottling, chlorosis, stunting, yellowing, and vein necrosis were collected from different fields in geographical regions of Maryland, Delaware, and Virginia. The virus survey was carried out using the enzyme-linked immunosorbent assay (ELISA) serological assay. The most common viruses of soybean with high infection rates include the Soybean mosaic virus (SMV, 19.35%), Soybean vein necrosis virus (SVNV, 10.85%), Alfalfa mosaic virus (AMV, 1.61%), Bean yellow mosaic virus (BYMV), and Bean pod mottle virus (BPMV). In addition, we studied the virus seed transmissibility of soybean under greenhouse and field conditions. We will verify the virus infection previously detected by ELISA by the highly sensitive RT-PCR method.