Historical Project Details: Modified Dietary Amino Acid Balance to Increase Soy Protein Inclusion in Trout Feeds and Validation of Calprotectin as a Biomarker of SBM Induced Intestinal Enteritis (2016)

2016

Modified Dietary Amino Acid Balance to Increase Soy Protein Inclusion in Trout Feeds and Validation of Calprotectin as a Biomarker of SBM Induced Intestinal Enteritis

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Contributor/Checkoff:

Soy Aquaculture Alliance

Category:

Export/Trade

Keywords:

Aquaculture

Parent Project:

This is the first year of this project.

Lead Principal Investigator:

Madison (Matt) Powell, University of Idaho

Co-Principal Investigators:

Project Code:

1640-512-5288

Contributing Organization (Checkoff):

United Soybean Board

(n/a)

Institution Funded:

University of Idaho

(n/a)

Final Report Final Report 2

Brief Project Summary:

There was no effect of soybean meal level or amino acid supplementation level on final fish weights. Protein retention efficiency was depressed by high soy inclusion and further depressed with excessive amino acid supplementation. The study found that soybean meal is detrimental to production efficiency of rainbow trout at the 40% inclusion level in the diet. Current ideal amino acid targets may be in excess and need to be revised. There are detrimental effects to over-supplementing rainbow trout diets with amino acids. Molecular analyses of intestinal tissues and feces were able to isolate nine genes from rainbow trout.

Key Audience:
Aquaculture nutritionists, aquaculture feed producers, trout producers

Information And Results

Project Deliverables

The impacts of soybean meal on fish performance are quite variable. For some species, formulating diets based on digestible nutrients to include protein, amino acids and digestible energy can ensure a high utility of soybean meal.

Output:
In this study we examined amino acid inclusion levels and showed that current ideal amino acid targets may be in excess and that adding excess amino acids can have detrimental and interactive effects in diets with high soy inclusion levels in rainbow trout. These data allow feed formulators to further refine amino acid targets when supplementing soybean meal in rainbow trout diets.

Unfortunately, high levels of soybean meal also commonly affect gut health resulting in an inflammatory response with a complex milieu of proteins being produced and many genes being expressed.

Output:
In this study, we further examined SA100 calcium-binding proteins that are commonly expressed during inflammation for use as biomarkers for soybean meal induced enteritis in rainbow trout. We determined that as many as nine SA100 protein genes are expressed but commercially available antibodies for the resulting proteins are not sufficiently discriminatory for use as non-destructive biomarkers. Further developmental research will need to be undertaken to synthesize specific antibodies for these proteins.

Both outputs directly address the SAA Program Area Priority of “Enabling Technologies to Advance Domestic Aquaculture”.

Final Project Results

Updated February 18, 2021:

View uploaded report PDF file

View uploaded report 2 PDF file

Results of the feeding trial showed there was no effect of soybean meal level or amino acid supplementation level on final fish weights. However, FCR was higher with 40% soybean meal inclusion and there was a detrimental interaction with high soy level and high AA supplementation. Feed intake results in this study were also similar. Moreover, protein retention efficiency was depressed by high soy inclusion and further depressed with excessive AA supplementation. These results address the first objective and clearly provide three conclusions:
1. Soybean meal is detrimental to production efficiency of rainbow trout at 40% inclusion in the diet.
2. Current ideal amino acid targets may be in excess and need to be revised.
3. There are detrimental effects to over-supplementing rainbow trout diets with amino acids.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.