2019
VIGS enabled interrogation of Soybean R Genes
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Contributor/Checkoff:
Ohio Soybean Council
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
VIGS-enabled interrogation of SB R Genes
Lead Principal Investigator:
Feng Qu, Ohio Agricultural Research and Development Center
Co-Principal Investigators:
Project Code:
19-R-03
Contributing Organization (Checkoff):
Ohio Soybean Council
$50,000
Institution Funded:
The Ohio State University
$50,000
Brief Project Summary:

Soybean aphids and the seedling rot pathogens Phytophthora sojae and Fusarium graminearum damage soybean health. Research strives to combat these diseases by searching soybean germplasm collections for disease-resistant/tolerant varieties and identifying the underlying genes responsible for resistance/tolerance. This project builds on previous studies to identify the actual gene(s) within each of the multi-gene loci that govern the resistance/tolerance. Use of virus-induced gene silencing (VIGS) vectors derived from bean pod mottle virus and apple latent spherical virus aid this process. This work is expected to accelerate the mobilization of these R genes to disease-susceptible varieties.

Key Benefactors:
farmers, agronomists, extension agents, soybean breeders, seed companies

Information And Results
Project Deliverables

First quarter (October 1 - December 31, 2018):
1. Characterization of transgenic soybean plants expressing the Rag5 gene.
2. Generation of ALSV-based VIGS constructs for Rps8 candidate genes.
3. Initiation of VIGS-assisted QRL interrogation.
4. Generation of AMV cDNAs in preparation for VIGS vector construction.
Second quarter (January 1 - March 31, 2019):
1. Functional evaluation of the Rag5-transgenic plants with aphid-feeding experiments.
2. Continued interrogation of the Rps8 locus with BPMV and ALSV VIGS vectors.
3. Initiation of functional analysis of significant genes within two QRLs.
4. Modification of AMV cDNAs to allow for incorporation of soybean gene fragments, prepare for VIGS testing.
Third quarter (April 1 - June 30, 2019):
Continue working on the investigations initiated during the first six months.
Fourth quarter (July 1 - September 30, 2019):
1. Complete the testing of the 16 candidate genes within the Rps8 locus.
2. Complete the characterization of at least two genes within the two QRLs.
3. Conclude the research on Rag5 by completing the characterization of transgenic soybean, publish the study.
4. Complete the development of the AMV-based VIGS system.

Final Project Results

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.