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Traditional control measures for IDC are "stack-able." For example, the effects of planting a resistant variety, an in-furrow application of FeEDDHA, and planting in wider rows, all "stack" and add to the reduction of IDC. A variety's resistance to IDC is determined by genes involved with iron uptake, and other genes involved with iron translocation or other processes. So, one could say that these genetic traits also "stack" to define the IDC resistance of a variety.

This research used this concept to identify potential control measures for IDC, on a physiological level. What happens when you "stack" a known control measure for IDC, FeEDDHA fertilizer, with other compounds that might reduce IDC? For example, IDC is, by definition, a lack of chlorophyll in the plant. What happens if you combine FeEDDHA with a compound that stimulates chlorophyll production by the plant?

This research screened seed treatments and foliar sprays to be used with FeEDDHA, to reduce IDC. Two examples are given here. In a greenhouse experiment, soil was treated with three rates of Fe as FeEDDHA (Soygreen), and the seed was treated with four rates of an experimental compound. The chlorophyll levels of the 1st and 2nd trifoliolate leaflets were measured. As anticipated, the two treatments "stacked" with each other, as both soil treatment with FeEDDHA and the seed treatment greatly increased the chlorophyll level of this susceptible variety, giving normal chlorophyll levels (>30) when both were used.

In a field study, chlorotic soybeans were sprayed with FeEDDHA, with and without an experimental compound. In each replicate, from left to right: No spray, sprayed with FeEDDHA, and sprayed with FeEDDHA plus an experimental compound. Again, the two compounds appeared to work together to reduce IDC. Further research is needed.

The experimental compounds are not identified in this report, as it is being determined if any of the treatments identified in this research could be patented by NDSU.