2019
High-Throughput Methods for Screening Soybean Varieties for Resistance to IDC
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Contributor/Checkoff:
North Dakota Soybean Council
Category:
Sustainable Production
Keywords:
GeneticsGenomicsSeed quality
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
R Jay Goos, North Dakota State University
Co-Principal Investigators:
Project Code:
QSSB
Contributing Organization (Checkoff):
North Dakota Soybean Council
$15,240
Institution Funded:
North Dakota State University
$15,240
Brief Project Summary:

Iron deficiency chlorosis is a destructive disorder of soybeans. Planting a resistant variety is the most important control measure. Other control measures, such as an in-furrow iron fertilizer, must be added to a resistant variety. Field screening is the most common method of evaluation of varieties for IDC resistance, but there remains a need for a more rapid greenhouse/growth chamber method. The objective of this research was to develop a practical greenhouse screening protocol, which could be used to identify varieties with high levels of resistance.

Key Benefactors:
farmers, agronomists, extension specialists, plant pathologists

Information And Results
Project Deliverables

The Principal Investigator is a frequent contributor to Extension efforts. The results will be presented in the appropriate print formats (Crop and Pest Report, North Dakota Soybean Grower Magazine, interviews with other outlets), the usual farmer/agronomist meetings (Soil and Water Workshop, etc.), and the scientific literature. The methods developed will be in the public domain, and can be used by researchers, agronomists, and seed companies.

Final Project Results

Update:

View uploaded report Word file

Iron deficiency chlorosis (IDC) remains as a widespread and destructive disorder of soybeans in North Dakota. Planting a resistant variety is the most important control measure. Other control measures, such as an in-furrow iron fertilizer, must be added to a resistant variety. Unfortunately, selection of a resistant variety is not as easy as it sounds. The commercial life-span of a variety is typically only 3 or 4 years, and the information given to farmers by many seed companies, about the IDC resistance of their varieties, is often not accurate. NDSU performs field testing for IDC resistance, but only a small fraction of the marketplace gets tested. The objective of this project was to develop a rapid (~4 week) greenhouse screening procedure that would identify the top-end of IDC resistance.

The procedure developed involves growing individual plants in conical pots, about 6" long ("Conetainers"), in a 1:1 soil:sand mixture, that is sub-irrigated to keep the soil quite wet, as in the field. The soil is from a field location that consistently produces IDC in soybeans. Chlorosis is intensified by adjusting the concentration of nitrate in the sub-irrigation solution, and by the rate of phosphorus applied to the soil.

The final protocol involved a nitrate sub-irrigation solution of 10 mmol sodium nitrate, and 100 mg of phosphorus per 2 kg of soil:sand mixture. It was not necessary to include bicarbonate in the sub-irrigation solution. An example of the results are shown in Figure 1. From left to right, there is Iowa State germplasm A11, a very resistant line, Traill, which has acceptable IDC resistance, and Glacier and Stine 0480, which do not have acceptable levels of IDC resistance.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.