Updated March 31, 2020:
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Soilborne pathogens can reduce soybean yield and quality. Limited research has been conducted in recent years to characterize and identify problematic fungal pathogens to species. Project objectives included: (1) Characterize fungal pathogens in Mid-Atlantic conventional and organic soybean production and observe the frequency of isolation across farms. (2) Build a collection of isolates that can be screened for fungicide sensitivity and used in other projects to verify pathogen species using molecular protocols. (3) Identify locations with high disease pressure that may be used for future research and demonstration plots. In 2019, sixty field sites were surveyed. Soilborne fungal pathogens were isolated from 40% of sampled fields. Three species of the fungus Diaporthe were isolated from stem and root tissue across 17% of fields, along with Macrophomina phaseolina, the causal agent of charcoal rot, isolated from 17% of fields. A subset of twenty-six isolates were selected for in-vitro fungicide efficacy to three fungicides. All fungi screened were sensitive to all products with ED50 values ranging from <0.01 – 2.4 ppm. From this trial, a field was identified that will be used for soybean seed treatment efficacy trials in 2020, other field sites with Diaporthe species present were identified for continued survey work in 2020.