2018
Using Rhizobacteria for Nematode Control
Contributor/Checkoff:
Category:
Sustainable Production
Keywords:
NematodePest
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Chris Taylor, The Ohio State University
Co-Principal Investigators:
Project Code:
18-R-18
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:

The use of biocontrol agents for nematode control has become more prevalent. Previous work identified numerous plant-growth promoting rhizbacteria isolated from soil and root samples that exhibit biocontrol activity against root-knot and cyst nematodes. This research examines the quality of nematode control under diverse soil conditions and determines potential effects on yield and disease control. The project aims to finish evaluating the Pseudomonas strain collections for SCN activity, test colonization potential of plant roots of each strain, determine if SCN-active strains exhibit plant-growth promotional activity and conduct microplot field trials to test specific Pseudomonas strains against a Ohio SCN population.

Key Benefactors:
farmers, agronomists, extension agents

Information And Results
Project Deliverables

Final Project Results

Update:
We have finished the analysis of all microplot data. Over 600 microplots were used in our study across three growing seasons using 15 different treatments. We have tested individual and combinations of Pseudomonas for SCN control. We have found that even though modest SCN control was obtained (15-25% reduction), the level of control was deemed not enough to warrant development of a commercial product. We also continued mode of action studies for our Pseudomonas. One area we are currently exploring is whether some of our SCN-active strains produced volatiles that are toxic to nematodes. Experiments are focused on testing whether addition of substrates for volatile production leads to improved volatile activity. We have identified that hydrogen cyanide and methane thiols (and related compounds) were produced by our nematode-active Pseudomonas and that these volatiles can impact plant-pathogenic nematode and fungi. Current work is focused on examining how to boost production of these volatiles in the soil to help reduce nematode numbers.

We have finished the analysis of all microplot data. Over 600 microplots were used in our study across three growing seasons using 15 different treatments. We have tested individual and combinations of Pseudomonas for SCN control. We have found that even though modest SCN control was obtained (15-25% reduction), the level of control was deemed not enough to warrant development of a commercial product. We also continued mode of action studies for our Pseudomonas. One area we are currently exploring is whether some of our SCN-active strains produced volatiles that are toxic to nematodes. Experiments are focused on testing whether addition of substrates for volatile production leads to improved volatile activity. We have identified that hydrogen cyanide and methane thiols (and related compounds) were produced by our nematode-active Pseudomonas and that these volatiles can impact plant-pathogenic nematode and fungi. Current work is focused on examining how to boost production of these volatiles in the soil to help reduce nematode numbers.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.