2018
VIGS-enabled Interrogation of SB R Genes
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Contributor/Checkoff:
Ohio Soybean Council
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
VIGS-enabled interrogation of SB R Genes
Lead Principal Investigator:
Feng Qu, Ohio Agricultural Research and Development Center
Co-Principal Investigators:
Project Code:
18-R-03
Contributing Organization (Checkoff):
Ohio Soybean Council
$50,000
Institution Funded:
The Ohio State University
$50,000
Brief Project Summary:

Soybean aphids and the seedling rot pathogens Phytophthora sojae and Fusarium graminearum damage soybean health. Research strives to combat these diseases by searching soybean germplasm collections for disease-resistant/tolerant varieties and identifying the underlying genes responsible for resistance/tolerance. This project builds on previous studies to identify the actual gene(s) within each of the multi-gene loci that govern the resistance/tolerance. Use of virus-induced gene silencing (VIGS) vectors derived from bean pod mottle virus and apple latent spherical virus aid this process. This work is expected to accelerate the mobilization of these R genes to disease-susceptible varieties.

Key Benefactors:
farmers, agronomists, extension agents, soybean breeders, seed companies

Information And Results
Project Deliverables

We expect to pin down the Rag5R gene and, time permitting, produce seeds of Rag5-expressing transgenic soybean in a high yielding variety that is originally aphid-susceptible.
Using VIGS constructs based on BPMV, ALSV, and CPSMV, we expect to complete the evaluation of the 16 Rps8 candidate genes using VIGS, and to determine the resistance potential of these genes. Completion of this objective will lay the foundation for further characterization of additional R loci.
We expect this series of QRL interrogation to extend the usefulness of VIGS to the analysis of genes in QRLs, allowing us to not only identify genes responsible for QRL traits, but also investigate their mode of functions.
The virus-mediated a vr expression procedure will be successfully established and optimized, providing breeders with a faster and more reliable assay for selecting R gene-containing progeny of crosses

Final Project Results

Update:
See attachment

View uploaded report PDF file

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.