Aim 1. Stacking of EPA fatty acid, astaxanthin, and high vitamin E antioxidant traits into a single soybean line for optimized aquaculture feed using synthetic biology tools.
Aim 2. Improved omega-3 EPA and total oil content in Nebraska soybean germplasm.
Aim 3. Improvement of astaxanthin pigment levels in Nebraska soybean germplasm.
Aim 4. Evaluation of new oil traits in aquaculture feeding studies.