2018
Enhancing soybean germplasm through biotechnology
Contributor/Checkoff:
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Lead Principal Investigator:
Tom Clemente, University of Nebraska at Lincoln
Co-Principal Investigators:
Project Code:
750
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:

This research program brings together a multidisciplinary approach for the creation and evaluation of novel input and output traits in soybean. These include a gene stack combination to enhance photosynthetic capacity, and combining these photosynthesis genes with genes expressed specifically during seed development designed to increase oil percentage, while maintaining protein quality and quality. More recently, we designed and introduced into soybean genetic constructs that are expected to alter stomata counts in leaf surfaces as a unique means to improve drought tolerance in the crop. Importantly, this program translates the novel soybean created from greenhouse to field environments,...

Unique Keywords:
#breeding & genetics
Information And Results
Project Deliverables

Phenotyping under field conditions of the selected lead events carrying the respective transgenes for photosynthesis boost, stacked with a subset oil genes will be conducted 2017, with greenhouse and small scale testing initiated with transgene stacks for the complete set of photosynthesis genes and lipid genes, in both lanceolate leaf and conventional genetic backgrounds in 2018. The goal being the creation of soybean transgenic lineages that harbor transgenes to boost photosynthesis while enhancing sink strength towards oil, without compromising protein.

Preliminary characterization of the soybean events carrying transgenes to alter stomata number will be carried out during along with physiological data acquisition during 2017, with small scale field plot studies in 2018.

SCN screening with the Giesler group on the transgenic soybean events carrying the AQP transgene will continue through 2017, with field trials planned for 2018 to monitor impact of the AQP transgenic allele on agronomic performance under both irrigated and non-irrigated conditions. With respect to the baking oil component of the program, during the 2017 and 2018 growing seasons we will conduct expanded trials to permit data collection on various agronomic traits and
allow for securing of sufficient oil for down-stream testing for baking applications.

The aphid control component of the program will entail screening of the transgenic events carrying the new genetic element designed to simultaneously target four critical genes in the insect during 2017. If successful in observing an enhanced aphid resistance phenotype relative to the single and double gene target vectors, small plots will be planted in the field during 2018.

Final Project Results

Updated January 23, 2019:

View uploaded report PDF file

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.