Objective 1 deliverables.
Arkansas:
• Licensing the released high sucrose and low stachyose germplasm line, UA 5515HS in 2017.
• Re-evaluate and release a second-high sucrose and low stachyose line, R13-10658 by summer 2018.
• Genetic mapping to identify and confirm genes for sucrose, stachyose, and protein and study the interaction among these traits by fall 2017.
• Advanced lines with modified soluble carbohydrates (RS2-W331) and other desired seed quality traits and evaluate them in USDA Soybean Uniform test.
• Enter advanced high sucrose and low stachyose lines into the USDA Soybean Uniform test in 2018.
• Incorporate Rs2 alleles into southern-adapted germplasm by 2019.
Rainey:
• Soybean germplasm and advanced breeding lines with high sucrose (>7%) and low RFOs will be evaluated annually and released based on the best agronomic traits and carbohydrate profiles.
• Advanced lines entered into the USDA Soybean Uniform test in 2019, and annually thereafter.
• Germplasm for selection of breeding lines with modified soluble carbohydrates derived from diverse sources and using more than one mutation developed.
• Germplasm for selection of breeding lines with modified soluble carbohydrates plus higher protein developed.
Chen:
• New lines with improved sugar, low oligosaccharide from Chen’s breeding program will be evaluated annually and those lines with best agronomic traits and desired carbohydrate profiles will be released as germplasm lines or germplasm.
• Enter advanced lines into the USDA Soybean Uniform test in 2018.
Nguyen:
• Upon breeders’ sample nomination following the harvest season, MAS will be accordingly performed and reported to the breeders timely late 2017 and early 2017.
• From 100-150 selected progenies/breeding lines will be genotyped for each project cooperator.
• Each quarter of the FY 2017, 1,200-1,500 seed samples will be accurately phenotyped for carbohydrate composition and timely reported to the project PI as well as cooperators.
• Enhancement of NIR calibration for sucrose content through collaboration with breeders and Lorenz lab.
Hudson:
• November 2017 – Genotype pools and individuals for mutations
• Mutant seed ready for sucrose phenotyping
• June 2018 – mutants ready for planting
Bilyeu:
• Select and advance additional NILs with the variant allele combinations of the RS2 (W331-) and RS3 (snp6) genes that will target 200 pounds of each line to be available in early 2018 for Dr. Brake.
• -January 2017-provide 100 pounds of down-regulated rs2 and control NIL line to Dr. Brake
• -Select and advance additional NILs with the variant allele combinations of the RS2 (W331-) and -RS3 (snp6) genes that will target 200 pounds of each line to be available in January 2018 for Dr. Brake.
• -January 2018-identification of >8% seed sucrose level from mutant lines.
Lorenz:
• The advanced breeding populations and breeding lines will continue to be progressed through the breeding program until a germplasm line release decision is made.
• Enter advanced lines into the USDA Soybean Uniform test in 2018.
• Move Rs2 into MG 0-1 germplasm by 2019.
Objective 2 deliverables.
Brake:
• For all lines submitted for feeding studies and on an annual basis, a ME value index will be developed for each SBM relative to a control. This ME index will encompass both animal live performance, TME, and digestibility data. In addition, for each line in each feeding study, a value for kcal/lb. will be quantified that will serve as a target for improved soybean meal. Continue evaluation of economic value of identified ME increment relative to specific cultivars will be undertaken. Feedback will be provided to breeders.
Rainey:
• Annually, promote the most valuable lines in the project for quantification of metabolizable energy and the component pricing value of modified carbohydrate soybean meal.
• Produce seed for feeding studies to attempt to quantify the energy contribution of protein by conducting feeding studies with lines that have higher protein and typical soluble carbohydrate profiles.
Brake and Rainey:
• By 2019, determine if modified soluble carbohydrate meal can provide 100 kcal/lb. of energy above commodity.
AR, Lorenz, Rainey, Chen:
• The best lines developed by breeders will be increased and promoted for pilot projects based on performance data from USDA Uniform Tests.
Objective 3 deliverables.
Rainey:
• By 2019, translate trait interactions and measures of stability in the nutritional bundle into practical economic targets for soluble carbohydrates soybean breeders.
• By 2019, make recommendations about the contribution of high sucrose to meal value.
• By 2019, interpret feeding study results in terms of achievable seed composition and quality.
• In 2017, initiate populations that combine known high sucrose QTL.
• In 2017, 45,000 pounds each of four lines produced.
• Seed needed for 2018 treated and bagged.
• In 2018, Seed needed for feeding studies sent for processing.
Brake:
• Quantify value index of meal using data from with NILs, and data from lines with modified soluble carbohydrates, plus HOLL or higher protein.
Nguyen:
• A subset of approx. 10 exotic germplasm with environmental protein and carbohydrate stability will be identified in late 2016.
• Several Dr. Chen’s F5 progenies will be identified and confirmed for the germplasm and germplasm line development in 2017.
• Several SNPs significantly associated with high sucrose QTL will be also identified and utilized for the marker development.
• Candidate causal genes of high sucrose can be detected in these QTL regions. The gene-based functional genetic markers will be developed for MAS.
• Two sucrose mapping populations (KB07-15 x PI 229343; SA12-1455 x PI 417015 and a diverse panel of 350 PI lines of the USB sequencing project will be phenotyped for genetic analysis and association mapping in 2017 by the Nguyen lab.
• For each mutant gene of rs2 and kti, NIL sets can be developed for each MG anticipated in summer 2017.
• Similarly, one or two NIL sets for high sucrose content can be developed based on the completion of QTL mapping anticipated in summer 2017.
• From the QTL mapping and GWAS performed in early 2017, either known QTL conferring high sucrose will be confirmed or novel QTL can be detected and genetically mapped in the soybean chromosomes.
Hudson:
• November 2017 – rs2 W331 rs3 G75E seed ready for phenotyping
• December 2017 - fad2–1a fad2-1b rs2 rs3 F1 advanced in greenhouse
Bilyeu:
• Supply MG II germplasm lines to Dr. Rainey for final selection in 2018.
• MG III/IV lines will have 200 pounds of each NIL available in early 2019 for Dr. Brake.
Rainey, Bilyeu, Lorenz, and AR:
• Two generations annually of NIL development in a range of maturity groups: Lorenz 0, I; Rainey II, III; Bilyeu III, IV; AR V.
• MG 0-V BC2S3 lines will be increased in 2019 for future studies.
Rainey and Lorenz:
• Develop a data set for prediction and association mapping of soluble carbohydrates in the USDA germplasm collection by 2018.
• By 2019, evaluate trait interactions (protein, seed size, etc.) with soluble carbohydrates in publicly available data sets and germplasm resources- SoyNAM, USDA germplasm collection, and/or USB core germplasm resequencing project.
Lorenz:
• An association panel will be grown in 2017 and 2018. Results from this study will help understand the genetic architecture on the traits described.