1. Identification of the source of carbon for non-digestible carbohydrates produced late in development by studying metabolic flux in wild type and oligosaccharide-deficient lines
2. Generation of 3-5 homozygous/near-homozygous RNAi soybean lines that will be studied in year 2 and also can serve as a background for further modifications
3. Molecular phenotyping of 10 lines from fast neutron mutant population to identify a subset relevant to proposal goals and to advance characterization of existing soybean collections
4. 3-5 candidate genes identified from combined year 1 analyses for development of advanced lines in year 2 based on carbon partitioning results and oligosaccharide production hypotheses