2018
Improving CRISPR gene editing in soybean
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Contributor/Checkoff:
Iowa Soybean Association
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Steve Whitham, Iowa State University
Co-Principal Investigators:
Project Code:
Contributing Organization (Checkoff):
Iowa Soybean Association
$82,773
Institution Funded:
Iowa State University
$82,773
Brief Project Summary:

The development of CRISPR-based technologies has created unprecedented opportunities for editing the genes of crop plants to create optimized versions of genes that can be used in breeding programs. This project directly addresses the development and demonstration of CRISPR technologies to expand the sites that can be targeted for mutagenesis in the soybean genome or that can increase the precision by which we can edit the target genes. The goal is to expand the CRISPR toolkit for soybean. To test ideas about base editors, this work targets specific genes with known precise mutations to produce sulfonylurea or glyphosate herbicide resistance, so these will be ideal genes for proof-of-concept studies.

Key Benefactors:
farmers, agronomists, Extension agents, soybean breeders, seed companies

Information And Results
Project Deliverables

1. Create constructs for efficiently expressing CRISPR/Cas9 base editor and CRISPR/Cpf1 in soybean
2. DNA base edits in Als1 and Als2 genes using the CRISPR/Cas9 base editor – hairy roots
3. DNA base edits in Als1 and Als2 genes using the CRIPR/Cas9 base editor – transformed soybean plants
4. Heritable edits in Als1 and Als2 genes that confer resistance to sulfonylurea herbicide
5. Gene edits in eIF4E and eIFiso4E genes using CRISPR/Cpf1 – hairy roots
6. Gene edits in eIF4E and eIFiso4E genes using CRISPR/Cpf1 – transformed soybean plants
7. Heritable edits in eIF4E and eIFiso4E genes that confer resistance to Soybean mosaic virus

Final Project Results

Update:

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.