2017
Characterization and enhancement of soybean genetic resources for soilborne disease resistance
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Contributor/Checkoff:
North Central Soybean Research Program
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
Characterization and enhancement of soybean genetic resources for soilborne disease resistance
Lead Principal Investigator:
James Kurle, University of Minnesota
Co-Principal Investigators:
Jianxin Ma, Purdue University
Aaron Lorenz, University of Minnesota
Nevin Young, University of Minnesota
+2 More
Project Code:
NCSRP
Contributing Organization (Checkoff):
North Central Soybean Research Program
$190,212
Institution Funded:
University of Minnesota
$190,212
Brief Project Summary:

The goals of this project are to characterize, identify, isolate , and utilize novel genes/QTLs conferring complete resistance or partial resistance to P. sojae, P. ultimum P. irregulare, and F. graminearum. The new sources of resistance will then be deployed for effective disease management in the North Central region in collaboration with the USB-funded soybean disease research project. We have four research objectives that will help us achieve these goals

Unique Keywords:
#breeding & genetics, #genetic soilborn disease
Information And Results
Project Deliverables

(1) Markers/QTLs for partial resistance to root rot caused by P. sojae, F. graminearum, P. ultimum, and P. irregulare. Markers/ QTLs associated with resistance to seed rot and damping off caused by P. ultimum and P. sojae. Gene-based markers specific to RpsUN1 and RpsUN2.
(2) Early maturity lines or pre-breeding experimental lines resistant to P. sojae, F. graminearum, P. ultimum, and P. irregulare that can be used in breeding commercial soybean cultivars adapted to northern soybean growing areas.

Final Project Results

Update:
Objective 1:
Phenotyping of 280 ancestral lines for resistance to F. gramearum, P.ultimum, and P. irregulare seed and seedling rot was completed, root necrosis, root length, root dry weight measured and recorded for all lines for all pathogens.
Objective 2:
(Minnesota) Data recorded, evaluated for quality and control of variability (such as removal of outliers), and organized into datasets for statistical analysis and association mapping to identify markers for resistance. (Purdue) RpsUN1 and RpsUN2 candidate genes pinpointed by a combination of fine-mapping and candidate gene expression analysis.
Objective 3:
Progeny seed from introgression of RpsUN1 and RpsUN2 into four Purdue elite cultivars harvested. These seeds were planted in greenhouse for one generation of self-pollination. We completed analysis of the haplotypes of the two candidate genes using the SNP genotyping data available for the entire USDA soybean germ plasm collection.
Objective 4:
(Minnesota) Progeny seed of crosses of RpsUN1 and RpsUN2 with early maturity elite breeding lines planted for winter increase in Chilean nursery.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.