2016
Increasing Soybean Oil Yield by Targeted Silencing of a Novel Negative Regulator of Fatty Acid Synthesis (1620-632-6601)
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Contributor/Checkoff:
United Soybean Board
Category:
Sustainable Production
Keywords:
(none assigned)
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Jay Thelen, University of Missouri
Co-Principal Investigators:
Project Code:
1620-632-6601
Contributing Organization (Checkoff):
United Soybean Board
(n/a)
Institution Funded:
University of Missouri
(n/a)
Brief Project Summary:

Unique Keywords:
#gene silencing,soybean oil, fatty acid synthesis, #seed composition
Information And Results
Project Deliverables

1. A binary vector RNA interference construct capable of reducing expression of all isoforms of BADC genes in any soybean variety through RNA interference. This construct will contain gene sequences specific to BADC isoforms 1 and 2 in tandem as part of a coupled RNAi gene silencing strategy. We have used this gene silencing strategy in Arabidopsis as proof-of-principle.

2. Transgenic soybean lines with significantly (t-test, one-way ANOVA) higher levels of seed oil than nontransgenic reference lines.

Final Project Results

We will determine if silencing the BADC genes in soybean enhance soybean seed composition traits

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.