For objective 1
1. Identify new sources of SCN resistance
2. Release conventional cultivars and germplasm lines with SCN resistant in MG I, II, and III (Cianzio, ISU)
3. Release soybean germplasm lines with SCN and RKN resistance in maturity groups VI. VII and VIII (Arelli, USDA and Zenglu Li, UGA)
4. Improved germplasm lines with SCN resistance from Peking source
5. Improved diagnostic DNA marker assays for selection of SCN (rhg1 and rhg4, and RKN resistance
For objective 2
1. The development of several mapping populations derived from new sources of resistance to SCN, RKN, and RN.
2. Completion of QTL analyses of RKN and RN resistance in the Magellan x PI 567516C population.
3. Completion of QTL analyses of SCN, RKN and RN resistance in the new population derived from the Magellan x PI 567305 population.
4. Completion of the evaluation of over 500 PIs from early MG for SCN resistance.
5. Completion of association mapping study using 50k SNP Infinium Chip data to detect novel genomic regions conveying resistance to SCN.
6. Narrowing down the genomic regions of novel QTL by fine-mapping.
7. The development of molecular markers associated with the genes/QTL identified and test high-throughput SNP genotyping platform using the Fluidigm EP1 system.
8. Robust SNP marker assays for selection of root-knot Ma and Mj resistance
For objective 3
1. Soybean lines with resistance to HG Type 2.5.7 derived from G. tomentella.
2. Soybean lines with resistance to HG Type 1-7 derived from G. tomentella.
3. Mapping populations that can be used to identify the genomic location of SCN resistance genes from G. tomentella.