2016
Engineered resistance to soybean cyst nematode via induced gene silencing (RNAi)
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Contributor/Checkoff:
North Central Soybean Research Program
Category:
Sustainable Production
Keywords:
NematodePest
Parent Project:
Engineered resistance to soybean cyst nematode via induced gene silencing (RNAi)
Lead Principal Investigator:
Harold Trick, Kansas State University
Co-Principal Investigators:
Tim C. Todd, Kansas State University
Lila Vodkin, University of Illinois at Urbana-Champaign
+1 More
Project Code:
BG4505
Contributing Organization (Checkoff):
North Central Soybean Research Program
(n/a)
Institution Funded:
Kansas State University
(n/a)
Brief Project Summary:

The primary goal of this research project is to establish a new set of biotech traits that have durable resistance to SCN (Heterodera glycines). Turning off genes by a process known as RNA interference (RNAi) has tremendous potential as a new strategy towards nematode resistance. In the free-living nematode Caenorhabditis elegans, which serves as a model, specific genes can be selectively turned off in the nematode by either injecting or feeding specific small interfering (si) RNA molecules to the worms. We propose to use this approach to introduce siRNA molecules into soybean so that SCN will take up these small molecules upon feeding, providing a selectively lethal meal. Depending on the...

Unique Keywords:
#biotechnology, #nematodes, #resistance, #scn, #soybean cyst nematode
Information And Results
Project Deliverables

We anticipate SCN bioassays should result in the identification of specific lines with enhanced SCN resistances. Correlating bioassay results and the population of siRNA within individual transgenic plants will allow us to predict how much siRNA target sequences are required to be effective in the suppression of SCN. Using different HG types in bioassays and reapplying nematodes from a single population on a continuous basis should answer whether the transgenes can provide durable resistance to diverse populations and HG types.

Final Project Results

Update:
See final report under October 3rd above.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.