2016
Development of soybean genotypes with enhanced capacity of nitrogen fixation
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Contributor/Checkoff:
North Central Soybean Research Program
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Stella Kantartzi, Southern Illinois University
Co-Principal Investigators:
Project Code:
Contributing Organization (Checkoff):
North Central Soybean Research Program
(n/a)
Institution Funded:
Southern Illinois University at Carbondale
(n/a)
Brief Project Summary:

The objective of our proposed study is to obtain new soybean cultivars and breeding lines with enhanced BNF capacity. These genotypes will be derived from RIL populations, which will be developed aiming to combine genes for high-yielding potential and improved N fixation. These RIL populations will also used to identify QTL associated with traits that are directly and indirectly related to BNF.
The development of the new RIL populations will be accomplished by crossing soybean genotypes possessing distinct N fixation capacities. These genotypes have been already screened for their overall N fixation capacity in our preliminary experiments, and they are plant introductions (PIs), Japanese,...

Unique Keywords:
#breeding & genetics, #breeding for nitrogen fixation, #qtl mapping for nitrogen fixation, #traits for nitrogen fixation
Information And Results
Project Deliverables

• Parental crossing in the greenhouse: Divergent soybean genotypes have been identified and characterized in the preliminary experiments for the desirable traits. They will be crossed in the greenhouse to obtain the required amount of F1 seeds for developing RIL populations.
• F1 planting in the greenhouse: The hybrid seeds will be sown in greenhouse and the required amount of F2 seeds will be obtained from each crossing combination.
• F2 to F4 advancement via shuttle breding: Generation advancement from the F2 to F4 will be performed in Puerto Rico.
• Field evaluation and selection: Advanced populations will be planted, evaluated, and lines will be selected under field conditions in southern Illinois.
• Evaluation of RIL populations: Combined selected lines will integrate each RIL population and those will be screened and phenotyped for the traits of interest.
• Genotyping of RIL populations: Each line from the RIL populations will be genotyped using SSR and SNP markers.
• Analysis and results: Phenotypic and genotypic data will be analyzed for QTL mapping and superior breeding lines will be selected.

Final Project Results

Update:
See final report under October 3, 2016

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.