Historical Project Details: Genes and Markers for Resistance to Phytophthora Sojae, Pythium spp., and Fusarium Graminearum in Soybean (Year 2 of 1420-532-5659) (2015)

2015

Genes and Markers for Resistance to Phytophthora Sojae, Pythium spp., and Fusarium Graminearum in Soybean (Year 2 of 1420-532-5659)

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Contributor/Checkoff:

United Soybean Board

Category:

Sustainable Production

Keywords:

(none assigned)

Parent Project:

Genes and Markers for Resistance to Phytophthora Sojae, Pythium spp., and Fusarium Graminearum in Soybean (Year 1 of 1420-532-5659)

Lead Principal Investigator:

Anne Dorrance, The Ohio State University

Co-Principal Investigators:

Chris Taylor, Danforth Plant Science Center
Alison Robertson, Iowa State University
Feng Qu, Ohio Agricultural Research and Development Center
Leah McHale, The Ohio State University
Henry Nguyen, University of Missouri
Grover Shannon, University of Missouri
Michelle Graham, USDA/ARS-Iowa State University
Rouf Mian, USDA/ARS-Ohio State University
M A Saghai Maroof, Virginia Tech

+8 More

Project Code:

1520-532-5659

Contributing Organization (Checkoff):

United Soybean Board

(n/a)

Institution Funded:

The Ohio State University

(n/a)

Final Report

Brief Project Summary:

Unique Keywords:
#breeding & genetics, #fusarium species, #phytophtora sojae, #pythium spp., #soybean disease resistance

Information And Results

Project Deliverables

1. Characterize and fine map sources of potentially novel Rps genes towards Phytophthora sojae.
Germplasm/varieties/markers associated with novel Rps genes for Phytophthora sojae.
Numerous plant introductions from the soybean germplasm collection have been identified with potentially novel Rps genes.
2. Using Virus Induced Gene Silencing (VIGS) to assay the function of candidate P. sojae Rps genes.
Within these loci, we have identified numerous candidate R-genes. It is important to identify the correct sequence to facilitate rapid development of new varieties. Thus, our collective goal is to fine map these novel R-gene loci, compare R-gene sequences, and verify the activity using virus induced gene silencing.
3. Identify QTL to those that are essential for resistance towards seed and seedling pathogens: Phytophthora sojae, Pythium spp., and Fusarium graminearum.
4. Fine map QTL and identify key genes involved in the expression of resistance in these populations.
5. Functional analysis of key genes associated with partial resistance to P. sojae, Pythium spp. and F. graminearum.
For these 3 objectives/strategies, we will have developed molecular markers that are directly tied to genes associated with the resistance response to these key soybean seed and root pathogens. In addition, we will have available, germplasm with key QTL to use in variety development, varieties for release from different backgrounds will be in progress.
In addition we will have identified numerous sources of resistance to the several key pathogens and will have populations, genetic maps of these populations for others to screen and map other traits.
6. Development of highly adapted germplasm with QTL identified in these studies.
Develop and release germplasm and varieties with resistance to multiple soybean root pathogens.
7. Assess the population diversity of Phytophthora sojae and key Pythium spp. throughout the Midwest, targeted towards identifying key pathotypes or effectors required for screening.
Characterized pathogenisolate collections that can be used for screening and comparison of resistance response.

Final Project Results

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.