2013
Soybean Disease Resistance Signaling Networks in Response to Soybean Cyst Nematode (Year 2 of 2268)
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Contributor/Checkoff:
United Soybean Board
Category:
Sustainable Production
Keywords:
(none assigned)
Parent Project:
Soybean Disease Resistance Signaling Networks in Responding to Soybean Cyst Nematode (Year 1 of 2268)
Lead Principal Investigator:
Melissa Goellner Mitchum, University of Missouri
Co-Principal Investigators:
Khalid Meksem, Southern Illinois University
Project Code:
1320-532-5668
Contributing Organization (Checkoff):
United Soybean Board
(n/a)
Institution Funded:
University of Missouri
(n/a)
Brief Project Summary:

Unique Keywords:
#nematodes
Information And Results
Project Deliverables

1) Complementation analysis of the Rhg4 gene: hairy root and stable soybean transformation under the control of native promoter. This experiment will unequivocally demonstrate that we have isolated the gene conferring resistance to SCN at the Rhg4 locus.
2) Characterize the Rhg4 gene: gene function, regulation and expression upon SCN infection. These experiments will help us determine exactly how this gene is functioning to confer resistance against SCN. 3) Investigate interactions between the Rhg4 gene and soybean genes/products. This experiment will enable us to identify additional soybean genes that may be functioning in the Rhg4-mediated resistance signaling pathway.
4) Test the function of potential soybean genes found to interact with the Rhg4 gene. These experiments will confirm additional plant components of the Rhg4-mediated signaling pathway.
5) Investigate interactions between the Rhg4 gene and nematode genes/products. This experiment will help us identify which genes in the nematode encode proteins that are detected by resistant soybean plants to trigger the resistance response.
6) Test the function of potential nematode genes found to interact with the Rhg4 gene. These experiments will aid in identifying the proteins used by virulent SCN to overcome or evade the Rhg4-mediated resistance response.

Final Project Results

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.