2012
Application of Biotechnology to Control of the Soybean Cyst Nematode: SCN Parasitism Genes (Year 3 of 0214)
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Contributor/Checkoff:
United Soybean Board
Category:
Sustainable Production
Keywords:
(none assigned)
Parent Project:
Application of Biotechnology to Control of the Soybean Cyst Nematode: SCN Parasitism Genes (Year 3 of 7214)
Lead Principal Investigator:
Thomas Baum, Iowa State University
Co-Principal Investigators:
Eric Davis, North Carolina State University
Melissa Goellner Mitchum, University of Missouri
+1 More
Project Code:
0214
Contributing Organization (Checkoff):
United Soybean Board
(n/a)
Institution Funded:
Iowa State University
(n/a)
Brief Project Summary:

Unique Keywords:
#nematodes
Information And Results
Project Deliverables

- Quantifying the native expression level of each nematode parasitism gene to identify how the gene expression level influences the RNAi effect.
- Identify the copy number of each parasitism gene in the SCN genome and determine how expression of each gene can influence RNAi effect.
- Determine not only the effect of PG-RNAi on the number of cyst females produced on host roots, but identify potential effects on the length of the nematode life cycle and the number of eggs produced per nematode.
- Quantify the expression level of PG-RNAi expressed in plants and compare this to target native nematode gene expression levels to identify the correct dose of RNAi needed to maximize nematode inhibition.
- Analyze the RNAi transgene constructs used to transform plants to optimize maximum effective RNAi production at the site of nematode infection in roots.
- Target multiple nematode parasitism genes with RNAi in the same transgenic plant to identify combinations that provide very high levels of nematode resistance.

Final Project Results

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.