The goal of this project is to evaluate genetic strategies to increase total oil content of soybean to greater than 30%. To this end we are testing two approaches to perturb carbon flow in developing soybean seeds as a means to enhance the oil/protein ratio in mature seed. In addition we will characterize transgenic soybeans that possess a four gene stack for the production of the very-long chain omega-3 fatty acid, EPA and a separate three gene stack in transgenic soybean for the production of the carotenoid, astaxanthin.