With the release of the soybean genome in 2008, a tremendous amount of information has become available on soybean genes and the adjacent DNAs that regulate those genes. For the proposed research, 20 genes associated with defense response to various pathogens will first be selected. The promoter regions of these genes, which are in front of the genes and control their expression, will be isolated and characterized. As these defense genes are typically turned on, in response to pathogen exposure/invasion, regions of DNA within the promoter that are responsible for its regulation, will be identified and characterized. To perform promoter analysis, we will utilize gene expression validation...
Unique Keywords:
#breeding & genetics, #molecular engineering, #soybean breeding, #soybean disease resistance