Improve soybean nodulation by beneficial rhizobacteria and thereby improve the nitrogen-fixing ability of desirable soybean cultivars. Our research has identified four candidate genes that regulate specificity in soybean-rhizobacteria interactions. Silencing either of these genes (two glycerol-3-phosphate dehydrogenase isoforms, LY1a, b, and two membrane-associated proteins NDR1 & LEA) promotes soybean nodulation by incompatible strains of rhizobacteria. In the past year, we generated constructs for RNAi-mediated silencing of the two GLY1 genes and submitted them to the University of Missouri-Columbia plant transformation facility for transfer into soybean. The goal is to generate stable...
Unique Keywords:
#nitrogen fixation, #rnai strategies, #seed composition, #soybean nodulation