Update:
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When taking Y25 E13P1 and P3 events as examples, we found that different plants from a single event have very varied expression levels according to the reporter, especially for T1 generation seeds which is segregating. As RT-qPCR working on the reporter, it will be the first guide to select high expression transgenic plants for offspring seeds of other transgenic lines later. So far, it is likely that stable transgenic lines Y25E13, Prp17 01-03P6 and J23 E4P1 have significant affects on SCN development, and could be one of good resources for SCN resistance. Other potential lines from initial screen test are under the way, and several next generations of seeds were produced too. The lines with significant SCN reduction will be tested again for replications.