The research team has established a demographics bioassay of hairy roots using hatched SCN eggs. This demographics bioassay system will be useful to rapidly investigate whether RNAi constructs containing SCN genes have effects for suppressing SCN growth in the soybean roots. The researcher team performed SCN bioassays for stable transgenic plants using SCN HG type 7 infected soils. Bioassays indicated that there was a 35% reduction in SCN eggs on the transgenic lines compared to the controls.

In related experiments, they constructed artificial micro RNAs vectors with three potential target genes and vectors that express two of these targets at the same time. For our research on root...