Two small plot experiments were established in Central Iowa at the Agronomy & Ag Engineering and Bruner research Farms. Hardwood biochar was purchased from a commercial producer (Royal Oak) and applied at the rates of 0, 10, 20, 30, and 40 t/acre.
A series of laboratory and greenhouse experiments was conducted in order to understand the mechanism responsible for SDS suppression by biochar. As previously observed in our studies suppressive effect of biochar on SDS disease severity can be attributed to systemic acquired resistance (SAR) induced by organic chemicals present in biochar, or by direct inhibition of germination, reproduction and survival rates of Fusarium virguliforme.
A laboratory experiment examined the direct effect of water-soluble chemical compounds leached from biochar on growth of F. virguliforme. The biochar extracts had no effect on spore germination compared to the water control. A greenhouse experiment is currently underway in order to evaluate the effect of organic or inorganic compounds released from biochar as potential inducers of SAR using a split-root technique.