2014
Mapping and introgression of RpsN for resistance of Phytophthora root rot into elite soybean lines
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Contributor/Checkoff:
Iowa Soybean Association
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
Mapping and introgression of RpsN for resistance of Phytophthora root rot into elite soybean lines
Lead Principal Investigator:
Alison Robertson, Iowa State University
Co-Principal Investigators:
Madan Bhattacharyya, Iowa State University
Silvia Cianzio, Iowa State University
+1 More
Project Code:
Contributing Organization (Checkoff):
Iowa Soybean Association
(n/a)
Institution Funded:
Iowa State University
(n/a)
Brief Project Summary:

The goal of this research is to develop elite soybean lines with improved resistance to Phytophthora sojae, the pathogen that causes Phytophthora root rot. In a previous ISA funded project mapping QTLs for partial resistance to P. sojae, we identified a potential novel gene for resistance to this pathogen. The goal of this proposal is to develop elite soybean lines with improved resistance to P. sojae, the causal organism of Phytophthora root rot.

The researchers identified the RpsN gene region is located on Chromosome 3 (MLG N). SNP markers flanking the gene region was identified but were not tightly linked. SSR markers are being explored to cover the gaps in the genome maps and...

Unique Keywords:
#breeding & genetics, #genetic resistance to diseases, #phytophtora root rot, #soybean gene markers, #soybean germplasm screening
Information And Results
Project Deliverables

1. Generate molecular markers linked to RpsN.
2. Backcross RpsN into elite soybean germplasm lines.
3. Identify candidate RpsN genes.

Final Project Results

• Screening of the two RIL populations with P. sojae isolate R17 (1b,1d,3a,3b,3c,5,6) and combined R17 and Val12-11 (1a,1b,1c,1d,1k,2,4,7) isolates resulted in 2 genes and a single gene segregation, respectively.
• Populations were screened with twelve polymorphic SSR markers identified in the parental lines and 3 were successfully mapped in one of the RIL populations.
• Genetic maps were regenerated and the mapping was conducted using ICIMapping software.
• F2 seeds of the AX20925 population are being screened to determine the gene action.
• DNA pools of the resistant and susceptible RIL are currently being sequenced.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.