1. Map quantitative trait loci (QTLs) for low RFOs, higher sucrose and low trypsin inhibitor using four RIL (recombinant inbred line) populations. These populations were developed using the new sources for the above traits discovered in PIs lab.
2. Develop breeder-friendly DNA markers for use in marker-assisted selection.
3. Select breeding lines with low raffinose oligosaccharides, high sucrose and low trypsin inhibitor from mapping and breeding populations for germplasm release.