The goal of this project is to develop superior soybean varieties possessing low oligosaccharides without comprising other exiting good traits such as protein and oil yields. There are several specific milestones we will achieve: 1) design and construct transformation vectors that will not only enable to obtain single-copy and marker-free transgenic soybean but also reduce soybean oligosaccharides efficiently, 2) develop 40 transgenic soybean plants using the above constructs and select plant lines displaying the above desirable traits, 3) propagate sufficient seeds of 1-2 desirable soybean lines for processing, animal feeding study. Success in this project can be measured by the degree to which these 3 milestones are achieved.