2011
Reducing Soybean Yield Loss by Enhancing Resistance to Phytophthora Rot (Year 1 of 1291)
Contributor/Checkoff:
Category:
Sustainable Production
Keywords:
(none assigned)
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Aardra Kachroo, University of Kentucky
Co-Principal Investigators:
Project Code:
1291
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:

Unique Keywords:
#phytophthora root and stem rot, soybean diseases, mechanism for disease resistance, #soybean diseases
Information And Results
Project Deliverables

Milestones
1. Generating the various silencing and overexpressing constructs for GmRIN- months 3-4 (Year 1)
2. Generating the various silencing and overexpressing constructs for GmNDR- months 3-4 (Year 1)
3. Silencing/overexpressing the different combinations of the GmRIN/GmNDR genes using the above constructs in soybean- months 12-24 (Years 1-2)
4. Analysis of effects of silencing/overexpressing GmRIN/GmNDR on resistance to P. sojae race 1- months 12-24 (Years 2-3)
5. Analysis of effects of silencing/overexpressing GmRIN/GmNDR on resistance to various different races of P. sojae - months 12-36 (Years 1-3)
6. Studying interactions between P. sojae virulence/avirulence proteins and GmRIN – months 12-24 (Years 2-3).

Final Project Results

Updated October 30, 2017:
This project was recently (Feb 2011) renewed for an additional 3 years with the objective of
resistance to Phytophtora elucidating the role of two plant defense genes on Phytophthora resistance, GmRIN and GmNDR. A BPMV-based vector for silencing both GmNDR (a & b) isoforms was generated and inoculated on soybean plants. Plants simultaneously silenced GmNDRa & b were obtained. Silencing of the two genes was confirmed by RT-PCR analysis. Construction of BPMV silencing vector targeting all four GmRIN genes is underway.
Also, a BPMV-based vector for over expressing GmRINb was generated and inoculated on soybean plants. Plants over expressing GmRINb were obtained. Over expression of GmRINb was confirmed by RT-PCR analysis

This project is still in the middle of generating results that foster the development of improved tools for disease resistance. Yet, some of the results thus far are useful for laying the groundwork for research and development in this area.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.