The goal of the project was to increase quality seeds of high-protein soybean lines that we developed in order to have sufficient seeds (5 pounds each) of 150 high-protein lines. The lines had seed protein content from about 55% to 60% on a dry matter basis. The current calibration that is available on NIR machines at university and USDA research labs and at points of sale for soybean producers does not cover the range of protein that we have achieved in our breeding program. We have developed soybean lines with the highest protein percentage that has been reported for soybean seeds. The problem for further research and breeding progress is that the calibration plateaus at about 54% protein,...