FY21: KPI (1) Generation of soybean customized constructs to overexpress PsbS under drought conditions; the first quarter (delivered). KPI (2) Generation of TO transgenics; the third quarter (initiated). KPI (3) The propagation of TO to T2 seeds, along with segregation for homozygosity and functionality of the introduced T-DNA; the fourth quarter and if needed will be continued in the first quarter of FY22. FY22 and FY23: KPI (4) Evaluation of T2 lines for drought-inducible overexpression of PsbS and water savings under drought conditions in greenhouse settings; the second quarter of FY22. KPI (5) The physiological, molecular, and growth characterization of selected T2 lines under controlled and drought conditions in greenhouse settings; the third quarter of FY22. KPI (6) The characterization of transgenics in the field; the third and the fourth quarters of FY22 and FY23.
This project will have significantly positive impact to soybean farmers by leading to new soybean variety which can diminish the 4O% lost in soybean yield which is annually caused by drought.
The results will be published in an academic journal and in public formats, including UNL websites and local news, to disseminate the water-saving advantages of the generated soybean lines to the public.