We anticipate the completion of the project genetic selection will improve feed efficiency by 10-15% per generation which means plant protein consumption will increase and greatly decrease the cost of trout production (Objective #1). We envisage selecting the most feed efficient trout families by measuring the metabolic rate (Objective #3). We expect each individual fish microbiome can be linked to detailed individual-level phenotypes of growth, feed efficiency, and nutrient turnover, as measured using stable-isotope profiling. Ultimately it will demonstrate an association between the microbial composition in the distal gut and a key aspect of trout metabolism. This association could be a direct effect of microbes contributing to improved nutrient availability and absorption for the host (Objectives # 2 and 4). Overall, genetic improvement increases farm profit, either via cost reduction per unit product, increased production output, or a combination of both. Improving nutrient efficiency would also have a positive effect on the environmental impact of fish farming. The outcomes will contribute to start the genetic improvement program by producing brood stock of rainbow trout with the traits of best SBMD utilization efficiency, which is good news for the aquaculture industry including the fish farmers, and feed industry especially the soybean producer.